Posted On : Feb 18, 2020
Updated On : Feb 18, 2020
Expires On : Dec 14, 2020
ace c18 hplc column
ACE C18 is the optimum and most versatile starting point for most method development activities. It offers ultra-inert silica for superior peak shape and guaranteed batch-to-batch and column-to-column reproducibility. ACE C18 provides hydrophobic interactions and some shape selectivity for general reversed-phase applications and analytes including those differing in hydrophobicity, uncharged acids and bases, polar to non-polar analytes and ionised acids and bases (using ion-pair chromatography).
ACE C18-AR combines the hydrophobic characteristics of a C18 phase with the enhanced aromatic selectivity of a phenyl phase, allowing the benefits of both interactions to be fully exploited. The phase also exhibits ultra low bleed to ensure LC-MS compatibility and demonstrates excellent temperature and pH stability. Additionally, ACE C18-AR is suitable for use in highly aqueous (up to 100%) mobile phases to enable the retention and separation of polar compounds. It is useful for analytes with electron withdrawing and delocalised systems such as nitro groups, ketones, esters, acids and aromatic compounds.
ACE C18-PFP combines the hydrophobic characteristics of a C18 phase with the alternative selectivity offered by a pentafluorophenyl phase, providing pi-pi, dipole-dipole, hydrophobic and shape selectivity mechanisms. This phase is stable in 100% aqueous mobile phase conditions, exhibits ultra low bleed to ensure LC-MS compatibility and demonstrates excellent temperature and pH stability. ACE C18-PFP is ideal for halogenated analytes and analytes with electron donating groups such as phenols, aromatics, ethers and amines and is additionally useful for regioisomers.
ACE C18-Amide is a uniquely designed polar-embedded stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. The special design of the ACE C18-Amide ligand incorporates an extended alkyl chain spacer between the silica surface and amide polar group, which gives extra phase stability in eluents of pH 2 – pH 8. The longer C18 tail of the ligand increases the hydrophobic mechanism of interaction compared to phases with shorter (C14 – C16) tail groups. ACE C18-Amide offers low bleed for UV and LC-MS compatibility and can be used in 100% aqueous mobile phase conditions. This phase is recommended for polar to mid-polar analytes.
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